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CRISPR Gene Editing (eBook)

Methods and Protocols
Artikelnummer: 978-1-4939-9170-9
Einband: PDF
Verfügbarkeit: Download, sofort verfügbar (Link per E-Mail)
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This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPRgene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPRgene editing field.
Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.
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VerlagSpringer US
EinbandPDF
Erscheinungsjahr2019
Seitenangabe362 S.
AusgabekennzeichenEnglisch
AbbildungenXI, 362 p. 76 illus., 61 illus. in color.
Masse10'184 KB
Auflage19001 A. 1st ed. 2019
PlattformPDF
ReiheMethods in Molecular Biology; Springer Protocols
AutorLuo, Yonglun (Hrsg.)

Alle Bände der Reihe "Methods in Molecular Biology; Springer Protocols"

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